S-Adenosyl-L-Homocysteine Hydrolase（SAHH）

Homocysteine ( Hcy ) is a thiol-containing amino acid formed from methionine during S-adenosylmethionine-dependent transmethylation reactions . It has been demonstrated that even mild or moderately elevated levels of Hcy also increase the risk of atherosclerosis of the coronary , cerebral and peripheral arteries and cardiovascular disease . And currently the hcy level is regarded as the biomarker for cardiovascular disease diagnosis all over the world . 

S-adenosyl-L-homocysteine hydrolase (SAHH , EC 3.3.1.1) (AdoHcyase) is an enzyme of the activated methyl cycle, responsible for the reversible hydration of S-adenosyl-L-homocysteine into adenosine and homocysteine.

SAHH is a ubiquitous enzyme which binds and requires NAD⁺ as a cofactor. AdoHcyase is a highly conserved protein of about 430 to 470 amino acids. The family contains a glycine-rich region in the central part of AdoHcyase; a region thought to be involved in NAD-binding. This protein may use the morpheein model of allosteric regulation.

There are several methods for hcy level detection . And in all these methods the most time-saving , high sensitivity , high throughput is enzyme cycling methods . People has applied this method for hcy diagnosis kit , and this kit is very popular throughout the world .

The principle of the enzyme cycling methods is as the following :

 

Hcy:  Homocysteine                   SAM:    S-adenosyl-L-methionine HMT: Homocysteine methyltransferase     SAH:  S-adenosyl-L-homocysteine   SAHH: S-adenosyl-L-homocysteine hydrolase  ADA:  Adenosine  Deaminase

The SAHH we can provide is recombinant protein , and it is of high activity and good stability, which can be used for different applications .

Product description:

Appearance: White powder, lyophilized

Preservation solution: Tris buffer, pH8.0

Specific activity: 10U/mg

Unit Definition: One unit will catalyze 1.0 μmole of S-adenosyl-L-homocysteine to adenosine and Hcy per min at pH 7.4 at 37°C.

Basic information:

Cat#: Abt-P-1002                          

Source: Recombinant expression

pH Stability: pH6.0-8.0                  pI: 6.15

Molecular weight: About 44kDa (SDS-PAGE detection)

Purity: ≥90% (SDS-PAGE test)

Redissolution and storage: Redissolved in 20% glycerol, 4℃, store at -20℃ for long-term preservation, Avoid multiple freeze-thaw cycles.

Transportation: Cold packs 

Security: Only used for research, strictly prohibited from human or animal experiment.